Mouse monoclonal [MEM-75] antibody to Transferrin Receptor (Biotin).
Spécificité
This antibody reacts with an extracellular epitope of CD71 antigen, a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and T lymphocytes, macrophages, and erythroid precursors. It is lost on resting blood leukocytes. This antibody does not block binding of transferrin to the receptor.
Applications
Flow Cytometry, IP, ICC
Recommander des dilutions
Flow Cytometry: 2-5 µg/ml.
Reactivité
Human
Immunogène
NALM-6 human pre-B cell line.
Hôte
Mouse
Clonalité
Monoclonal
Clone
MEM-75
Isotype
IgG1
Conjuguer
Biotin
Purification
This antibody is conjugated with Biotin LC-NHS ester under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free Biotin.
Concentration
1 mg/ml
MW prédit
95 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Flow cytometric analysis of human PHA stimulated peripheral blood mononuclear cells stained with Anti-CD71 Antibody [MEM-75] (Biotin) (concentration in sample is 0.6 µg/ml, Streptavidin-APC).
Separation of cells stained with Anti-CD71 Antibody [MEM-75] (Biotin) (concentration in sample is 0.6 µg/ml, Streptavidin-APC, red-filled) from cells unstained by primary antibody (Streptavidin-APC, black-dashed) in flow cytometry analysis (surface staining).