Anti-Topoisomerase I Anticorps [ARC0708] (A307759)

Name: Anti-Topoisomerase I Antibody [ARC0708]
Brand: Antibodies.com
SKU: A307759
Price: 470.0 USD
Availability: InStock

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$470

Anticorps monoclonal [ARC0708] de lapin contre Topoisomerase I pour WB, IHC, ICC/IF et IP.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-Topoisomerase I Antibody [ARC0708]

Description du produit

Rabbit monoclonal [ARC0708] antibody to Topoisomerase I.

Applications

WB, IHC, ICC/IF, IP

Recommander des dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DNA topoisomerase I (TOP1) (P11387).

Séquence

MSGDHLHNDSQIEADFRLNDSHKHKDKHKDREHRHKEHKKEKDREKSKHSNSEHKDSEKKHKEKEKTKHKDGSSEKHKDKHKDRDKEKRKEEKVRASGDA

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC0708

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

100 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

DNA topoisomerase 1, DNA topoisomerase I, NUP98 fusion gene, TOP 1, TOP I, TOP1, TOP1_HUMAN, TOPI, Topoisomerase (DNA) I, Topoisomerase 1, Topoisomerase1, TopoisomeraseI, Type I DNA topoisomerase

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (7)

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Figure 1: Western Blot - Anti-Topoisomerase I Antibody [ARC0708] (A307759)

Western blot analysis of extracts of various cell lines, using Anti-Topoisomerase I Antibody [ARC0708] (A307759) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-Topoisomerase I Antibody [ARC0708] (A307759)

Western blot analysis of extracts of Mouse thymus, using Anti-Topoisomerase I Antibody [ARC0708] (A307759) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 3: Immunohistochemistry - Anti-Topoisomerase I Antibody [ARC0708] (A307759)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-Topoisomerase I Antibody [ARC0708] (A307759) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-Topoisomerase I Antibody [ARC0708] (A307759)

Immunofluorescence analysis of C6 cells using Anti-Topoisomerase I Antibody [ARC0708] (A307759) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).