Anti-TNF alpha Anticorps [MP6-XT22] (A281254)

Anti-TNF alpha Antibody [MP6-XT22] - BSA and Azide free

Rat monoclonal [MP6-XT22] antibody to TNF alpha.

This antibody recognises mouse tumor necrosis factor alpha (TNF alpha), a ~17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a ~26 kDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumor cell lines, and has an important immunoregulatory role.Rat anti Mouse TNF alpha antibody, clone MP6-XT22 is reported to neutralize the effects of mouse TNF alpha (Gaup et al. 2008 and Yang et al. 1995).

Flow Cytometry, ELISA, IHC-Fr, Functional Assays

ELISA: 1 µg/ml - 10 µg/ml, FC 1: 1:10 - 1:50, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Mouse, Rat

Recombinant mouse TNF alpha expressed in E. coli.

Rat

Monoclonal

MP6-XT22

IgG1

Protein G affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline.

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Rat anti Mouse TNF alpha antibody, clone MP6-XT22 recognizes mouse tumor necrosis factor alpha (TNF alpha), a ~17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a ~26 kDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumor cell lines, and has an important immunoregulatory role.Rat anti Mouse TNF alpha antibody, clone MP6-XT22 is reported to neutralize the effects of mouse TNF alpha (Gaup et al. 2008 and Yang et al. 1995).

APC1, APC1 protein, Cachectin, DIF, Differentiation inducing factor, Macrophage cytotoxic factor, soluble form, Tnf, TNF a, TNF macrophage derived, TNF monocyte derived, TNF superfamily member 2 , TNF superfamily, member 2, TNF, macrophage derived, TNF, monocyte derived, TNF-a, TNF-alpha, TNFA, TNFA_HUMAN, TNFSF2, Tumor necrosis factor, Tumor necrosis factor (TNF superfamily member 2) , Tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, Tumor Necrosis Factor, Membrane Form, Tumor necrosis factor, soluble form