Anti-TIMP1 Anticorps [M03] (A280983)

Anti-TIMP1 Antibody [M03]

Mouse monoclonal [M03] antibody to TIMP1.

This antibody recognises Tissue Inhibitor of Metalloproteinases 1 (TIMP-1) also known as metalloproteinase inhibitor 1, EPA and fibroblast collagenase inhibitor. TIMP-1 is a member of the TIMP family of proteins that assist in regulating the activity of matrix metalloproteinases (MMPs) and it is mainly expressed by macrophages and fibroblasts. TIMP proteins are specific endogenous inhibitors that bind to the active site of MMPs in a stoichiometric 1:1 molar ratio, thereby blocking access to ECM substrates (Kapoor et al. 2016, Robert et al. 2016). The TIMP proteins including TIMP-1 have poor specificity for any particular MMP and each can inhibit multiple MMPs with differing efficacies. The ratio of MMP and TIMP often determine the extent of ECM protein degradation and tissue remodeling. An imbalance of this ratio such as with decreased expression of TIMP can result in various pathological conditions, most notably, heart failure, osteoarthritis and cancer. Low levels of TIMP have also been associated with slow-to-heal wounds and venous wounds. The purified Mouse anti Human TIMP-1 antibody, clone M03 (MCA62678GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human TIMP-1 antibody, clone M05 (MCA6279B) as the detection antibody.

ELISA

ELISA: 1 µg/ml

Human

E. coli derived recombinant human full length TIMP-1 (Cys24-Ala207).

Mouse

Monoclonal

M03

IgG1

Unconjugated

Protein G affinity chromatography of tissue culture supernatant.

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human TIMP-1 antibody, clone M03, recognizes Tissue Inhibitor of Metalloproteinases 1 (TIMP-1) also known as metalloproteinase inhibitor 1, EPA and fibroblast collagenase inhibitor. TIMP-1 is a member of the TIMP family of proteins that assist in regulating the activity of matrix metalloproteinases (MMPs) and it is mainly expressed by macrophages and fibroblasts. TIMP proteins are specific endogenous inhibitors that bind to the active site of MMPs in a stoichiometric 1:1 molar ratio, thereby blocking access to ECM substrates (Kapoor et al. 2016, Robert et al. 2016). The TIMP proteins including TIMP-1 have poor specificity for any particular MMP and each can inhibit multiple MMPs with differing efficacies (Cui et al. 2017). The ratio of MMP and TIMP often determine the extent of ECM protein degradation and tissue remodeling. An imbalance of this ratio such as with decreased expression of TIMP can result in various pathological conditions, most notably, heart failure, osteoarthritis and cancer. Low levels of TIMP have also been associated with slow-to-heal wounds and venous wounds (Cui et al. 2017).The purified Mouse anti Human TIMP-1 antibody, clone M03 (MCA62678GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human TIMP-1 antibody, clone M05 (MCA6279B) as the detection antibody.

Clgi, Collagenase inhibitor, Collagenase inhibitor, Human, EPA, EPO, Erythroid Potentiating Activity, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, FLJ90373, HCI, Human Collagenase Inhibitor, Metalloproteinase inhibitor 1, Metalloproteinase inhibitor 1 precursor, OTTHUMP00000023214 , TIMP, TIMP 1, TIMP metallopeptidase inhibitor 1 , TIMP-1, TIMP1 protein, TIMP1_HUMAN, Tissue Inhibitor of Metalloproteinase 1, Tissue inhibitor of metalloproteinases, Tissue inhibitor of metalloproteinases 1, Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor