Figure 1: Western Blot - Anti-TIM22 Antibody (A305300)
Western blot analysis of extracts of various cell lines, using Anti-TIM22 Antibody (A305300) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Figure 2: Western Blot - Anti-TIM22 Antibody (A305300)
Immunoprecipitation analysis of 600µg extracts of rat brain cells using 3µg of Anti-TIM22 Antibody (A305300). This Western blot was performed on the immunoprecipitate using Anti-TIM22 Antibody (A305300) at a dilution of 1:500.