TIE2 (phospho Tyr1108) Cell Based Kit Elisa (A103810)

Nom du produit
TIE2 (phospho Tyr1108) Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This TIE2 (phospho Tyr1108) Cell Based ELISA Kit allows for the detection of TIE2 (phospho Tyr1108) and the effects that certain stimulation conditions have on TIE2 (phospho Tyr1108) expression in different cell lines. Qualitative determination of TIE2 (phospho Tyr1108) concentration is achieved by an indirect ELISA format. In essence, TIE2 (phospho Tyr1108) is captured by Anti-TIE2 (phospho Tyr1108) Antibodies while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the dye conjugated to the secondary antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. Anti-TIE2 Antibody is also included for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.
Plate-forme
Microplate
Méthode de détection
Fluorometric
Gamme de detection
> 5000 Cells
Reactivité
Human, Mouse
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
Angiopoietin 1 receptor, Angiopoietin-1 receptor, CD202b, CD202b antigen, EC 2.7.10.1, Endothelial tyrosine kinase, Endothelium specific receptor tyrosine kinase 2, hTIE 2, hTIE2, Hyk, p140 TEK, soluble TIE2 variant 1, soluble TIE2 variant 2, Tek, tek tyrosine kinase, TEK tyrosine kinase endothelial, TEK tyrosine kinase, endothelial, TIE 2, TIE2_HUMAN, Tunica interna endothelial cell kinase, Tyrosine kinase with Ig and EGF homology domains 2, Tyrosine kinase with Ig and EGF homology domains-2, Tyrosine protein kinase receptor TEK, Tyrosine protein kinase receptor TIE 2, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE 2, Tyrosine-protein kinase receptor TIE-2, Venous malformations multiple cutaneous and mucosal, venous malformations, multiple cutaneous and mucosal, VMCM, VMCM 1, VMCM1
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

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