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Anti-TGF beta 1 Anticorps (A92358)

Nom du produit
Anti-TGF beta 1 Antibody
Description du produit
Rabbit polyclonal antibody to TGF beta 1.
Applications
WB, IHC, ICC/IF
Recommander des dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivité
Human, Mouse, Rat
Immunogène
A synthetic peptide corresponding to a sequence within amino acids 291-390 of human TGF beta 1 (NP_000651.3).
Séquence
KNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
12 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
Camurati-Engelmann disease, Cartilage-inducing factor, CED, Differentiation inhibiting factor, DPD1, LAP, Latency-associated peptide, Prepro transforming growth factor beta 1, TGF beta, TGF beta 1 protein , TGF-beta 1 protein, TGF-beta-1, TGF-beta-5, TGF-beta1, TGFB, Tgfb-1, tgfb1, TGFB1_HUMAN, TGFbeta, TGFbeta1, Transforming Growth Factor b1, Transforming growth factor beta 1, Transforming growth factor beta 1a, transforming growth factor beta-1, transforming growth factor, beta 1, Transforming Growth Factor-ß1
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-TGF beta 1 Antibody (A92358) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-TGF beta 1 Antibody (A92358)
Western blot analysis of extracts of Mouse spleen, using Anti-TGF beta 1 Antibody (A92358) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry - Anti-TGF beta 1 Antibody (A92358) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Immunohistochemistry - Anti-TGF beta 1 Antibody (A92358)
Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-TGF beta 1 Antibody (A92358) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-TGF beta 1 Antibody (A92358) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunohistochemistry - Anti-TGF beta 1 Antibody (A92358)
Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-TGF beta 1 Antibody (A92358) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-TGF beta 1 Antibody (A92358) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunofluorescence - Anti-TGF beta 1 Antibody (A92358)
Immunofluorescence analysis of A-549 cells using Anti-TGF beta 1 Antibody (A92358) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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