Figure 1: Western Blot - Anti-TFIIB Antibody (A89343)
Western blot analysis of extracts of various cell lines, using Anti-TFIIB Antibody (A89343) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-TFIIB Antibody (A89343) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-TFIIB Antibody (A89343) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-TFIIB Antibody (A89343) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-TFIIB Antibody (A89343) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of L929 cells using Anti-TFIIB Antibody (A89343) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
![Flow Cytometry - Anti-TFIIB Antibody [PCRP-GTF2B-1D1] (A277638) - Antibodies.com](https://cdn.antibodies.com/image/catalog/277/A277638_1.jpg?profile=product_alternative)
![Flow Cytometry - Anti-TFIIB Antibody [PCRP-GTF2B-1D1] - BSA and Azide free (A278226) - Antibodies.com](https://cdn.antibodies.com/image/catalog/278/A278226_1.jpg?profile=product_alternative)
