Anti-TAF15 Anticorps [4D71] (A85450)

Name: Anti-TAF15 Antibody [4D71]
Brand: Antibodies.com
SKU: A85450
Price: 185.0 USD
Availability: InStock

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$185

Anticorps monoclonal [4D71] de souris contre TAF15 pour WB et ICC/IF.

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Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 4-6 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-TAF15 Antibody [4D71]

Description du produit

Mouse monoclonal (4D71 ) antibody to TAF15.

Applications

WB, ICC/IF

Recommander des dilutions

WB: 1:1,000, ICC/IF: 1:500-1:1,000

Reactivité

Human, Rat, Mouse

Immunogène

Recombinant full-length human TAF15, expressed in and purified from E. coli.

Hôte

Mouse

Clonalité

Monoclonal

Clone

4D71

Isotype

IgG1

Conjuguer

Unconjugated

Purification

Immunogen affinity purification.

Concentration

1 mg/ml

Masse moléculaire

56 kDa, 68 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

68 kDa TATA-binding protein-associated factor, Npl3, RBP56, RBP56_HUMAN, RNA binding protein 56, RNA-binding protein 56, TAF, TAF(II)68, TAF15 RNA polymerase II TATA box binding protein (TBP) associated factor 68kDa, TAF2N, TAFII68, TATA-binding protein-associated factor 2N

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Immunofluorescence - Anti-TAF15 Antibody [4D71] (A85450)

HeLa cell cultures stained with Anti-TAF15 Antibody (green) and Anti-Vimentin Antibody (A85421 | red). Anti-TAF15 Antibody reveals a granular nuclear localization typical of many RNA associated proteins. Blue is a DNA stain.

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Figure 2: Immunofluorescence - Anti-TAF15 Antibody [4D71] (A85450)

Immunofluorescent analysis of HeLa cells stained with Anti-TAF15 Antibody [4D71 ] (A85450), at a dilution of 1:500 in red, and co-stained with Anti-GAPDH Antibody (A85377), at a dilution of 1:1,000 in green. Blue is Hoechst staining of nuclear DNA. The Anti-TAF15 Antibody [4D71 ] (A85450) reveals TAF15 protein expressed exclusively in the nuclei of the cells, while Anti-GAPDH Antibody (A85377) produces strong cytoplasmic staining.

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Figure 3: Western Blot - Anti-TAF15 Antibody [4D71] (A85450)

Western blot analysis of cytosol or nuclear enriched fractions of cell line lysates using Anti-TAF15 Antibody [4D71] (A85450), at a dilution of 1:1,000, in green. The lanes contain samples of: [1] Protein standards, in red, [2] NIH-3T3 cytosol, [3] NIH-3T3 nuclear fraction, [4] HeLa cytosol, and [5] HeLa nuclear fraction. Double band at 56kDa and 68kDa detected predominantly in the nuclear enriched fraction corresponds to the TAF15 protein.

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Figure 4: Western Blot - Anti-TAF15 Antibody [4D71] (A85450)

Blot of 20 µg HeLa cell lysate (Lane 1) and 20 µg HEK293 cell lysate (Lane 2) was probed with Anti-TAF15 Antibody (1: 1,000). The Anti-TAF15 Antibody binds strongly and cleanly to a band at about 68 kDa coresponding to TAF15.