Figure 1: Western Blot - Anti-SYVN1 / HRD1 Antibody (A14100)
Western blot analysis of U2OS, using Anti-SYVN1 / HRD1 Antibody (A14100) at 1:2,500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.