Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human GPCR GPR14 protein to be purified and analysed in vitro.
Système d'expression
HEK293 cells
Nature
Synthetic
Espèces de protéines
Human
Longueur des protéines
Full length protein.
Masse moléculaire
Full length human GPCR GPR14 protein has a MW of 41.9 kDa.
Conjuguer
Unconjugated
Forme du produit
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nanodisc solubilization buffer (20mM Tris-HCl, 150mM NaCl, pH 8.0). Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Stockage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonymes
G protein coupled receptor 14, G-protein coupled receptor 14, GPR14, UR-2-R, UR-II-R, UR2R_HUMAN, Urotensin II receptor, Urotensin II receptor (UR II R), Urotensin-2 receptor, UTR, UTR2, Uts2r
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Figure 1: Synthetic Nanodisc Human GPCR GPR14 Protein (A317295)
ELISA plates were pre-coated with Synthetic Nanodisc Human GPCR GPR14 Protein (A317295) at 0.2µg/per well. Serial diluted Anti-Flag Monoclonal Antibody solutions were added, washed, and incubated with a secondary antibody before reading the ELISA. From the above data, the EC50 for Anti-Flag Monoclonal Antibody binding with Synthetic Nanodisc Human GPCR GPR14 Protein (A317295) is 10.47ng/ml.
