Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human DARC protein to be purified and analysed in vitro.
Système d'expression
HEK293 cells
Nature
Synthetic
Espèces de protéines
Human
Longueur des protéines
Full length protein.
Masse moléculaire
Full length human DARC protein has a MW of 35.6 kDa.
Conjuguer
Unconjugated
Forme du produit
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization.
Stockage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Figure 1: Synthetic Nanodisc Human DARC Protein (A325116)
Diagram showing how synthetic nanodiscs containing full-length multi-pass transmembrane proteins in a phospholipid bilayer are generated from native cell membranes.
