Anti-Syndecan-1 Antibody [DM45] - BSA and Azide free
Description du produit
Recombinant rabbit monoclonal [DM45] antibody to Syndecan-1.
Applications
ELISA, Flow Cytometry
Recommander des dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivité
Human
Hôte
Rabbit
Clonalité
Monoclonal
Clone
DM45
Isotype
IgG
Conjuguer
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Forme du produit
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Stockage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Notes générales
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Expi 293 cell line was transfected with irrelevant protein (A) and human CD138 (B) were surface stained with Anti-Syndecan-1 Antibody [DM45] - BSA and Azide free (A318650) at 1µg/ml followed by Anti-Rabbit IgG Antibody (Alexa 488).
Flow cytometry analysis of antigen binding of Anti-Syndecan-1 Antibody [DM45] - BSA and Azide free (A318650). (A) Anti-Syndecan-1 Antibody [DM45] - BSA and Azide free (A318650) does not bind to Jurkat cells that do not express CD138. (B) A clear peak shift of Anti-Syndecan-1 Antibody [DM45] - BSA and Azide free (A318650) was seen compared to the control when incubated with CD138-expressing MM.1S cells, indicating strong binding of Anti-Syndecan-1 Antibody [DM45] - BSA and Azide free (A318650) to CD138. Antibodies were incubated at 5µg/ml.
![Flow Cytometry - Anti-Syndecan-1 Antibody [DM45] - BSA and Azide free (A318650) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318650_1.jpg?profile=product_top)
![Flow Cytometry - Anti-Syndecan-1 Antibody [DM45] - BSA and Azide free (A318650) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318650_2.jpg?profile=product_top)
![Immunohistochemistry - Anti-Syndecan-1 Antibody [DM45] - BSA and Azide free (A318650) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318650_3.jpg?profile=product_top)
![Flow Cytometry - Anti-Syndecan-1 Antibody [DM45] - BSA and Azide free (A318650) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318650_1.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-Syndecan-1 Antibody [DM45] - BSA and Azide free (A318650) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318650_2.jpg?profile=product_top_thumb)
![Immunohistochemistry - Anti-Syndecan-1 Antibody [DM45] - BSA and Azide free (A318650) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318650_3.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-Syndecan-1 Antibody [DM45] - BSA and Azide free (A318650) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318650_1.jpg?profile=product_image)
![Flow Cytometry - Anti-Syndecan-1 Antibody [DM45] - BSA and Azide free (A318650) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318650_2.jpg?profile=product_image)
![Immunohistochemistry - Anti-Syndecan-1 Antibody [DM45] - BSA and Azide free (A318650) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318650_3.jpg?profile=product_image)
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![Immunohistochemistry - Anti-Syndecan 1 Antibody [SDC1/7180] - BSA and Azide free (A278377) - Antibodies.com](https://cdn.antibodies.com/image/catalog/278/A278377_1.jpg?profile=product_alternative)
![Flow Cytometry - Anti-Syndecan-1 Antibody [DM46] - Azide free (A318649) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318649_1.jpg?profile=product_alternative)
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