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Sumo1 Cell Based Kit Elisa (A103122)

Nom du produit
Sumo1 Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This Sumo1 Cell Based ELISA Kit allows for the detection of Sumo1 and the effects that certain stimulation conditions have on Sumo1 expression in different cell lines. Qualitative determination of Sumo1 concentration is achieved by an indirect ELISA format. In essence, the Sumo1 is captured by Anti-Sumo1 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this Sumo1 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
DAP1, GAP modifying protein 1, GAP-modifying protein 1, GMP 1, GMP1, OFC10, PIC 1, PIC1, SENP2, Sentrin, Sentrin 1, Small ubiquitin related modifier 1, Small ubiquitin-like modifier 1, Small ubiquitin-related modifier 1, SMT3, SMT3 homolog 3, SMT3 suppressor of mif two 3 homolog 1, SMT3, yeast, homolog 3, Smt3C, SMT3H3, Sumo 1, SUMO-1, SUMO1_HUMAN, Ubiquitin homology domain protein PIC1, Ubiquitin Like 1, Ubiquitin like protein SMT3C, Ubiquitin like protein UBL1, Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein SMT3C, Ubiquitin-like protein UBL1, UBL 1, UBL1
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Sumo1 Cell Based ELISA Kit (CB5665) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Sumo1 Cell Based ELISA Kit (CB5665)
Western blot analysis of extracts from 293 cells, using Anti-Sumo1 Antibody.
Protocol Diagram - Sumo1 Cell Based ELISA Kit (CB5665) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Sumo1 Cell Based ELISA Kit (CB5665)
A diagram showing the protocol for Sumo1 Cell Based ELISA Kit.
Protocol Summary - Sumo1 Cell Based ELISA Kit (CB5665) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Sumo1 Cell Based ELISA Kit (CB5665)
A summary of the protocol for Sumo1 Cell Based ELISA Kit.
Western Blot - Control Antibody - Sumo1 Cell Based ELISA Kit (CB5665) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - Sumo1 Cell Based ELISA Kit (CB5665)
The mouse monoclonal antibody to GAPDH is used as a positive control inSumo1 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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