Rabbit polyclonal antibody to STAT5 (phospho Tyr694).
Applications
WB, IHC
Recommander des dilutions
WB: 1:500-1:2,000, IHC: 1:100-1:200
Reactivité
Human, Mouse, Rat
Immunogène
A synthetic phosphorylated peptide around Y694 of human STAT5 (NP_003143.2/NP_036580.2).
Séquence
DGYVK
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer
Unconjugated
Purification
Affinity purification.
Masse moléculaire
90 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
MGF, Signal transducer and activator of transcription 5A, Signal Transducer and Activator of Transcription 5B, STA5A_HUMAN, STAT 5A, STAT 5B, STAT5A, STAT5B, Transcription factor STAT5A, Transcription factor STAT5B
Figure 1: Western Blot - Anti-STAT5 (phospho Tyr694) Antibody (A91399)
Western blot analysis of extracts of K562 cells, using Anti-STAT5 (phospho Tyr694) Antibody (A91399) at 1:1,000 dilution. K-562 cells were treated by ATP(5 mM) at 30°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.
Figure 2: Western Blot - Anti-STAT5 (phospho Tyr694) Antibody (A91399)
Western blot analysis of extracts of A-431 cells, using Anti-STAT5 (phospho Tyr694) Antibody (A91399) at 1:1,000 dilution or STAT5B antibody (A12356). A-431 cells were treated by EGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-STAT5 (phospho Tyr694) Antibody (A91399) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-STAT5 (phospho Tyr694) Antibody (A91399) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-STAT5 (phospho Tyr694) Antibody (A91399) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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