Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
MGF, Signal transducer and activator of transcription 5A, Signal Transducer and Activator of Transcription 5B, STA5A_HUMAN, STAT 5A, STAT 5B, STAT5A, STAT5B, Transcription factor STAT5A, Transcription factor STAT5B
Figure 1: Western Blot - Anti-STAT5 Antibody (A10213)
Western blot analysis of extracts of HeLa cells, using Anti-STAT5 Antibody (A10213) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunofluorescence analysis of C6 cells using Anti-STAT5 Antibody (A10213) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH/3T3 cells using Anti-STAT5 Antibody (A10213) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of U2OS cells using Anti-STAT5 Antibody (A10213) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
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