Western blot analysis of extracts of HeLa cells, using Anti-STAT1 Antibody (A92784) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using Anti-STAT1 Antibody (A92784) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180.
Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-STAT1 Antibody (A92784) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 200µg extracts of U-87MG cells using 3µg of Anti-STAT1 Antibody (A92784). This Western blot was performed on the immunoprecipitate using Anti-STAT1 Antibody (A92784) at a dilution of 1:1000.
Immunoprecipitation analysis of 200µg extracts of U-87MG cells using 3µg of Anti-STAT1 Antibody (A92784). This Western blot was performed on the immunoprecipitate using Anti-STAT1 Antibody (A92784) at a dilition of 1:500.