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SRY Cell Based Kit Elisa (A103753)

Nom du produit
SRY Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This SRY Cell Based ELISA Kit allows for the detection of SRY and the effects that certain stimulation conditions have on SRY expression in different cell lines. Qualitative determination of SRY concentration is achieved by an indirect ELISA format. In essence, the SRY is captured by Anti-SRY Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this SRY Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
Essential protein for sex determination in human males, Sex determining region on Y, Sex determining region protein, Sex determining region Y, Sex-determining region Y protein, sox21b, SRXX1, SRXY1, SRY 1, Sry1, Sry3BI, SRYGENE, SRY_HUMAN, TDF, TDY, Testis determining factor, Testis Determining Factor on Y, Testis-determining factor, Transcription factor Sox-21-B
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - SRY Cell Based ELISA Kit (CB5963) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - SRY Cell Based ELISA Kit (CB5963)
Western blot analysis of extracts from HeLa cells, using Anti-SRY Antibody. The lane on the right is treated with the synthesized peptide.
Protocol Diagram - SRY Cell Based ELISA Kit (CB5963) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: SRY Cell Based ELISA Kit (CB5963)
A diagram showing the protocol for SRY Cell Based ELISA Kit.
Protocol Summary - SRY Cell Based ELISA Kit (CB5963) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: SRY Cell Based ELISA Kit (CB5963)
A summary of the protocol for SRY Cell Based ELISA Kit.
Western Blot - Control Antibody - SRY Cell Based ELISA Kit (CB5963) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - SRY Cell Based ELISA Kit (CB5963)
The mouse monoclonal antibody to GAPDH is used as a positive control inSRY Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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