Anti-Src Antibody [ARC0378] (A307395)

Name: Anti-Src Antibody [ARC0378]
Brand: Antibodies.com
SKU: A307395
Price: 485.0 USD
Availability: InStock

Enlarge Image

$485

Rabbit monoclonal [ARC0378] antibody to Src for WB, ICC/IF and IP.

100% Guarantee

Price Match Guarantee

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Knockout Validated Antibody

Specifications

Name

Anti-Src Antibody [ARC0378]

Description

Rabbit monoclonal [ARC0378] antibody to Src.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Src (P12931).

Sequence

MGSNKSKPKDASQRRRSLEPAENVHGAGGGAFPASQTPSKPASADGHRGPSAAFAPAAAEPKLFGGFNSSDTVTSPQRAGPLAGGVTTFVALYDYESRTE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0378

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

p60-Src, pp60c-src, Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, SRC1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

Enlarge Image

Western Blot - Anti-Src Antibody [ARC0378] (A307395)

Western blot analysis of extracts of various cell lines, using Anti-Src Antibody [ARC0378] (A307395) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Enlarge Image

Western Blot - Anti-Src Antibody [ARC0378] (A307395)

Western blot analysis of extracts from wild type (WT) and Src knockout (KO) HeLa cells, using Anti-Src Antibody [ARC0378] (A307395) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1min.

Enlarge Image

Western Blot - Anti-Src Antibody [ARC0378] (A307395)

Western blot analysis of extracts of Jurkat cells, using Anti-Src Antibody [ARC0378] (A307395) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1min.

Enlarge Image

Immunofluorescence - Anti-Src Antibody [ARC0378] (A307395)

Immunofluorescence analysis of NIH-3T3 cells using Anti-Src Antibody [ARC0378] (A307395) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).