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Anti-SOX2 Anticorps (A81052)

Nom du produit
Anti-SOX2 Antibody
Description du produit
Rabbit polyclonal antibody to SOX2.
Applications
WB, IHC, ICC/IF
Recommander des dilutions
WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivité
Human, Mouse, Rat
Immunogène
Recombinant fusion protein containing a sequence within amino acids 135-215 of human SOX2 (NP_003097.1).
Séquence
GGNSMASGVGVGAGLGAGVNQRMDSYAHMNGWSNGSYSMMQDQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQT
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
35 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
ANOP3, cb236, Delta EF2a, lcc, MCOPS3, MGC148683, MGC2413, RGD1565646, Sex determining region Y box 2, SOX 2, SOX2_HUMAN, SRY (sex determining region Y) box 2, SRY box containing gene 2, SRY related HMG box 2, SRY related HMG box gene 2, SRY-box 2, Transcription factor SOX 2, Transcription factor SOX-2, ysb
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-SOX2 Antibody (A81052) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-SOX2 Antibody (A81052)
Western blot analysis of extracts of various cell lines, using Anti-SOX2 Antibody (A81052) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - Anti-SOX2 Antibody (A81052) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Immunohistochemistry - Anti-SOX2 Antibody (A81052)
Immunohistochemistry analysis of paraffin-embedded mouse fetal Brain using Anti-SOX2 Antibody (A81052) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-SOX2 Antibody (A81052) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunofluorescence - Anti-SOX2 Antibody (A81052)
Confocal immunofluorescence analysis of U-2 OS cells using Anti-SOX2 Antibody (A81052) at a dilution of 1:200. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-SOX2 Antibody (A81052) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunofluorescence - Anti-SOX2 Antibody (A81052)
Immunofluorescence analysis of C6 cells using Anti-SOX2 Antibody (A81052) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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