Anti-SOX10 Anticorps [PCRP-SOX10-1D8] (A250018)

Name: Anti-SOX10 Antibody [PCRP-SOX10-1D8]
Brand: Antibodies.com
SKU: A250018
Price: 525.0 USD
Availability: InStock

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$525

Anticorps monoclonal [PCRP-SOX10-1D8] de souris contre SOX10 pour IHC-P.

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Alternative Formats and ConjugatesAlternative Formats

Carrier Free and Preservative Free

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 4-6 jours ouvrables.

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Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Mots clés :

Anticorps Monospécifique Neuroepithelial Marker Oligodendrocyte Marker Schwann Cell Marker

Spécifications

Nom du produit

Anti-SOX10 Antibody [PCRP-SOX10-1D8]

Description du produit

Mouse monoclonal [PCRP-SOX10-1D8] antibody to SOX10.

Spécificité

The specificity of this monoclonal antibody to its intended target was validated by HuProt^TM Array, containing more than 19,000 full-length human proteins. Recognizes a protein of ~55kDa, identified as SOX10. This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. Expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Often used melanoma markers, such as anti-HMB45 and anti-MelanA, are poorly expressed in desmoplastic melanomas while SOX10 is moderately-to-strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes mammary glands. SOX-10 expression is also observed in mast cells.

Applications

IHC-P

Recommander des dilutions

IHC-P: 1-2 µg/ml

Reactivité

Human

Réactivité croisée

This antibody is predicted to cross react with Mouse and Rat.

Immunogène

Recombinant fragment, around amino acids 90-179, of human SOX10 protein. The exact sequence is proprietary.

Hôte

Mouse

Clonalité

Monoclonal

Clone

PCRP-SOX10-1D8

Isotype

IgG2b

Conjuguer

Unconjugated

Purification

Protein A/G chromatography.

Concentration

200 µg/ml

Masse moléculaire

49-58 kDa

Forme du produit

Liquid

Formulation

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes générales

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-SOX10 Antibody [PCRP-SOX10-1D8] - BSA and Azide free (A253198).

Synonymes

DOM, Dominant megacolon mouse human homolog of, MGC15649, PCWH, SOX 10, SOX10_HUMAN, SRY (sex determining region Y) box 10, SRY box 10, SRY box containing gene 10, SRY related HMG box gene 10, Transcription factor SOX 10, Transcription factor SOX-10, WS2E, WS4, WS4C

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Immunofluorescence - Anti-SOX10 Antibody [PCRP-SOX10-1D8] (A250018)

Immunofluorescent analysis of PFA fixed HeLa cells stained with Anti-SOX10 Antibody [PCRP-SOX10-1D8] followed by Goat Anti-Mouse IgG (CF® 488) (Green). Counterstain is Phalloidin (Red).

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Figure 2: Flow Cytometry - Anti-SOX10 Antibody [PCRP-SOX10-1D8] (A250018)

Flow cytometric analysis of PFA fixed HeLa cells using Anti-SOX10 Antibody [PCRP-SOX10-1D8] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Unstained cells (red).

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Figure 3: Anti-SOX10 Antibody [PCRP-SOX10-1D8] (A250018)

Analysis of protein array containing more than 19,000 full-length human proteins using Anti-SOX10 Antibody [PCRP-SOX10-1D8]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt^TM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt^TM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

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Figure 4: SDS-PAGE - Anti-SOX10 Antibody [PCRP-SOX10-1D8] (A250018)

SDS-PAGE analysis of Anti-SOX10 Antibody [PCRP-SOX10-1D8] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.