Anti-Sonic Hedgehog Anticorps (A285956)

Name: Anti-Sonic Hedgehog Antibody
Brand: Antibodies.com
SKU: A285956
Price: 480.0 USD
Availability: InStock

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$480

Anticorps polyclonal de chèvre contre Sonic Hedgehog pour ELISA, WB, IF et Flow Cytometry.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 3-5 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-Sonic Hedgehog Antibody

Description du produit

Goat polyclonal antibody to Sonic Hedgehog.

Applications

ELISA, WB, IF, Flow Cytometry

Recommander des dilutions

ELISA: 1:32,000, WB: 1-3µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivité

Human, Mouse

Immunogène

Synthetic peptide corresponding to the C terminal region of human Sonic Hedgehog.

Séquence

C-DSEALHPLGMAVK

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Masse moléculaire

48 kDa

MW prédit

49.6kDa

Forme du produit

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

HHG 1, HHG-1, HHG1, HLP 3, HLP3, Holoprosencephaly 3, HPE 3, HPE3, MCOPCB5, shh, SHH_HUMAN, SMMC I, SMMCI, Sonic Hedgehog (Drosophila) homolog, Sonic hedgehog homolog, sonic hedgehog homolog (Drosophila), Sonic hedgehog protein, Sonic hedgehog protein C-product, TPT, TPTPS

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (5)

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Figure 1: Western Blot - Anti-Sonic Hedgehog Antibody (A285956)

Sonic Hedgehog expression in Human Liver lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Sonic Hedgehog Antibody (A285956) at 2µg/ml and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-Sonic Hedgehog Antibody (A285956)

Sonic Hedgehog expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Sonic Hedgehog Antibody (A285956) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Plasma membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Immunofluorescence - Anti-Sonic Hedgehog Antibody (A285956)

Sonic Hedgehog expression in NIH3T3 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Sonic Hedgehog Antibody (A285956) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Plasma membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Immunofluorescence - Anti-Sonic Hedgehog Antibody (A285956)

Sonic Hedgehog expression in U251 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Sonic Hedgehog Antibody (A285956) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Plasma membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.