Anti-SMURF 2 Anticorps [ARC1897] (A308190)

Name: Anti-SMURF 2 Antibody [ARC1897]
Brand: Antibodies.com
SKU: A308190
Price: 470.0 USD
Availability: InStock

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$470

Anticorps monoclonal [ARC1897] de lapin contre SMURF 2 pour WB, IHC, ICC/IF et IP.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-SMURF 2 Antibody [ARC1897]

Description du produit

Rabbit monoclonal [ARC1897] antibody to SMURF 2.

Applications

WB, IHC, ICC/IF, IP

Recommander des dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivité

Human, Mouse

Immunogène

Recombinant fusion protein containing a sequence corresponding to amino acids 201-290 of human SMURF2 (Q9HAU4).

Séquence

PLSCFVDENTPISGTNGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSN

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC1897

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

80 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

E3 ubiquitin-protein ligase SMURF2, EC 6.3.2., hSMURF2, MGC138150, Smad specific E3 ubiquitin ligase 2, SMAD specific E3 ubiquitin protein ligase 2, SMAD ubiquitination regulatory factor 2, SMAD-specific E3 ubiquitin-protein ligase 2, SMUF2_HUMAN, Smurf2, Ubiquitin protein ligase SMURF2

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Western Blot - Anti-SMURF 2 Antibody [ARC1897] (A308190)

Western blot analysis of extracts of various cell lines, using Anti-SMURF 2 Antibody [ARC1897] (A308190) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Figure 2: Immunohistochemistry - Anti-SMURF 2 Antibody [ARC1897] (A308190)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-SMURF 2 Antibody [ARC1897] (A308190) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-SMURF 2 Antibody [ARC1897] (A308190)

Immunofluorescence analysis of NIH-3T3 cells using Anti-SMURF 2 Antibody [ARC1897] (A308190) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Western Blot - Anti-SMURF 2 Antibody [ARC1897] (A308190)

Immunoprecipitation analysis of 300µg extracts of mouse lung using 3µg of Anti-SMURF 2 Antibody [ARC1897] (A308190). This Western blot was performed on the immunoprecipitate using Anti-SMURF 2 Antibody [ARC1897] (A308190) at a dilution of 1:1000.