Anti-SMAD5 (phospho Ser465) Anticorps [ARC59204] (A309476)

Name: Anti-SMAD5 (phospho Ser465) Antibody [ARC59204]
Brand: Antibodies.com
SKU: A309476
Price: 540.0 USD
Availability: InStock

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$540

Anticorps monoclonal [ARC59204] de lapin contre SMAD5 (phospho Ser465) pour WB.

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Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-SMAD5 (phospho Ser465) Antibody [ARC59204]

Description du produit

Rabbit monoclonal [ARC59204] antibody to SMAD5 (phospho Ser465).

Applications

Recommander des dilutions

WB: 1:1,000-1:5,000

Reactivité

Mouse, Rat

Immunogène

A synthetic phosphorylated peptide around S465 of human Smad5.

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC59204

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

60 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

DKFZp781C1895, DKFZp781O1323, Dwfc, hSmad5, JV5 1, JV5-1, MAD homolog 5, MAD, mothers against decapentaplegic homolog 5, MADH 5, MADH5, Mothers against decapentaplegic homolog 5, mothers against decapentaplegic, drosophila, homolog of, 5, Mothers against DPP homolog 5, MusMLP, SMA and MAD related protein 5, SMAD 5, SMAD family member 5, SMAD, mothers against DPP homolog 5, SMAD5_HUMAN

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (2)

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Figure 1: Western Blot - Anti-SMAD5 (phospho Ser465) Antibody [ARC59204] (A309476)

Western blot analysis of NIH/3T3, using Anti-SMAD5 (phospho Ser465) Antibody [ARC59204] (A309476) at 1:2,000 dilution. NIH/3T3 cells were treated by BMP4(50ng/ml) at 37°C for 6 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-SMAD5 (phospho Ser465) Antibody [ARC59204] (A309476)

Western blot analysis of C6, using Anti-SMAD5 (phospho Ser465) Antibody [ARC59204] (A309476) at 1:2,000 dilution. C6 cells were treated by MG132(50 µM) and TGFbetaat 37°C for 90 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.