Anti-SMAD5 Antibody [ARC0448] (A308830)

Name: Anti-SMAD5 Antibody [ARC0448]
Brand: Antibodies.com
SKU: A308830
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC0448] antibody to SMAD5 for WB and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-SMAD5 Antibody [ARC0448]

Description

Rabbit monoclonal [ARC0448] antibody to SMAD5.

Applications

WB, IP

Dilutions

WB: 1:500-1:2,000, IP: 1:500-1:1,000

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Smad5 (Q99717).

Sequence

STYPNSPASSGPGSPFQLPADTPPPAYMPPDDQMGQDNSQPMDTSNNMIPQIMPSISSRDVQPVAYEEPKHWCSIVYYELNNRVGEAFHASSTSVLVDGFT

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0448

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

hSmad5, JV5-1, MAD homolog 5, MADH5, Mothers against decapentaplegic homolog 5, Mothers against DPP homolog 5, SMAD 5, SMAD family member 5

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-SMAD5 Antibody [ARC0448] (A308830)

Western blot analysis of extracts of K-562 cells, using Anti-SMAD5 Antibody [ARC0448] (A308830) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Western Blot - Anti-SMAD5 Antibody [ARC0448] (A308830)

Western blot analysis of extracts of C2C12 cells, using Anti-SMAD5 Antibody [ARC0448] (A308830) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Western Blot - Anti-SMAD5 Antibody [ARC0448] (A308830)

Immunoprecipitation analysis of 300µg extracts of K-562 cells using 3µg of Anti-SMAD5 Antibody [ARC0448] (A308830). This Western blot was performed on the immunoprecipitate using Anti-SMAD5 Antibody [ARC0448] (A308830) at a dilution of 1:1000.

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