Anti-Smad3 (phospho Thr179) Anticorps (A11032)

Name: Anti-Smad3 (phospho Thr179) Antibody
Brand: Antibodies.com
SKU: A11032
Price: 435.0 USD
Availability: InStock

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$435

Anticorps polyclonal de lapin contre Smad3 (phospho Thr179) pour WB et IHC.

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Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-Smad3 (phospho Thr179) Antibody

Description du produit

Rabbit polyclonal antibody to Smad3 (phospho Thr179).

Applications

WB, IHC

Recommander des dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic phosphorylated peptide around T179 of human Smad3 (NP_005893.1).

Séquence

PETPP

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

48 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

DKFZP586N0721, DKFZp686J10186, hMAD 3, hMAD-3, hSMAD3, HSPC193, HST17436, JV15 2, JV15-2, JV152, LDS1C, LDS3, MAD (mothers against decapentaplegic Drosophila) homolog 3, MAD homolog 3, Mad homolog JV15 2, Mad protein homolog, MAD, mothers against decapentaplegic homolog 3, Mad3, MADH 3, MADH3, MGC60396, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA and MAD related protein 3, SMAD, SMAD 3, SMAD family member 3, SMAD, mothers against DPP homolog 3, SMAD3_HUMAN

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (5)

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Figure 1: Western Blot - Anti-Smad3 (phospho Thr179) Antibody (A11032)

Western blot analysis of extracts of HeLa cell line, using Anti-Smad3 (phospho Thr179) Antibody (A11032). The lane on the right is treated with the antigen-specific peptide. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.

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Figure 2: Western Blot - Anti-Smad3 (phospho Thr179) Antibody (A11032)

Western blot analysis of extracts of PC-12 cells, using Anti-Smad3 (phospho Thr179) Antibody (A11032) at 1:1,000 dilution. PC-12 cells were treated by TNF-alpha (20 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunohistochemistry - Anti-Smad3 (phospho Thr179) Antibody (A11032)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Smad3 (phospho Thr179) Antibody (A11032) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Smad3 (phospho Thr179) Antibody (A11032)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Smad3 (phospho Thr179) Antibody (A11032) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.