Anti-Smad3 (phospho Ser423 + Ser425) Anticorps (A309475)

Name: Anti-Smad3 (phospho Ser423 + Ser425) Antibody
Brand: Antibodies.com
SKU: A309475
Price: 435.0 USD
Availability: InStock

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$435

Anticorps polyclonal de lapin contre Smad3 (phospho Ser423 + Ser425) pour WB.

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Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

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Spécifications

Nom du produit

Anti-Smad3 (phospho Ser423 + Ser425) Antibody

Description du produit

Rabbit polyclonal antibody to Smad3 (phospho Ser423 + Ser425).

Applications

Recommander des dilutions

WB: 1:500-1:1,000

Reactivité

Human, Mouse

Immunogène

A phospho synthetic peptide corresponding to residues surrounding S423/S425 of Human SMAD3.

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

55 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

DKFZP586N0721, DKFZp686J10186, hMAD 3, hMAD-3, hSMAD3, HSPC193, HST17436, JV15 2, JV15-2, JV152, LDS1C, LDS3, MAD (mothers against decapentaplegic Drosophila) homolog 3, MAD homolog 3, Mad homolog JV15 2, Mad protein homolog, MAD, mothers against decapentaplegic homolog 3, Mad3, MADH 3, MADH3, MGC60396, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA and MAD related protein 3, SMAD, SMAD 3, SMAD family member 3, SMAD, mothers against DPP homolog 3, SMAD3_HUMAN

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (2)

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Figure 1: Western Blot - Anti-Smad3 (phospho Ser423 + Ser425) Antibody (A309475)

Western blot analysis of extracts of various cell lines, using Anti-Smad3 (phospho Ser423 + Ser425) Antibody (A309475) at 1:1,000 dilution. Hela cells were treated by TGF-beta (10 ng/ml) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-Smad3 (phospho Ser423 + Ser425) Antibody (A309475)

Western blot analysis of extracts of various cell lines, using Anti-Smad3 (phospho Ser423 + Ser425) Antibody (A309475) at 1:1,000 dilution. C2C12 cells were treated by TGF-beta (10 ng/ml) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.