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SLP-76 (phospho Tyr128) Cell Based Kit Elisa (A103778)

Nom du produit
SLP-76 (phospho Tyr128) Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This SLP-76 (phospho Tyr128) Cell Based ELISA Kit allows for the detection of SLP-76 (phospho Tyr128) and the effects that certain stimulation conditions have on SLP-76 (phospho Tyr128) expression in different cell lines. Qualitative determination of SLP-76 (phospho Tyr128) concentration is achieved by an indirect ELISA format. In essence, SLP-76 (phospho Tyr128) is captured by Anti-SLP-76 (phospho Tyr128) Antibodies while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the dye conjugated to the secondary antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. Anti-SLP-76 Antibody is also included for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.
Plate-forme
Microplate
Méthode de détection
Fluorometric
Gamme de detection
> 5000 Cells
Reactivité
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
76 kDa tyrosine phosphoprotein, CG8697, LCP 2, LCP2, LCP2_HUMAN, Lymphocyte cytosolic protein 2, SH2 domain containing leukocyte protein 76 KD, SH2 domain containing leukocyte protein of 76kD, SH2 domain containing leukocyte protein of 76kDa, SH2 domain-containing leukocyte protein of 76 kDa, SLP 76, SLP 76 tyrosine phosphoprotein, SLP-76 tyrosine phosphoprotein, SLP76, SLP76 tyrosine phosphoprotein
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - SLP-76 (phospho Tyr128) Cell Based ELISA Kit (FLUO-CBP1885) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - SLP-76 (phospho Tyr128) Cell Based ELISA Kit (FLUO-CBP1885)
Western blot analysis of lysates from K562 cells treated with Na3VO4 0.3nM 40', using Anti-SLP-76 (phospho Tyr128) Antibody. The lane on the right is blocked with the phospho peptide.
Protocol Diagram - SLP-76 (phospho Tyr128) Cell Based ELISA Kit (FLUO-CBP1885) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: SLP-76 (phospho Tyr128) Cell Based ELISA Kit (FLUO-CBP1885)
A diagram showing the protocol for SLP-76 (phospho Tyr128) Cell Based ELISA Kit.
Protocol Summary - SLP-76 (phospho Tyr128) Cell Based ELISA Kit (FLUO-CBP1885) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: SLP-76 (phospho Tyr128) Cell Based ELISA Kit (FLUO-CBP1885)
A summary of the protocol for SLP-76 (phospho Tyr128) Cell Based ELISA Kit.
Western Blot - Control Antibody - SLP-76 (phospho Tyr128) Cell Based ELISA Kit (FLUO-CBP1885) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - SLP-76 (phospho Tyr128) Cell Based ELISA Kit (FLUO-CBP1885)
The mouse monoclonal antibody to GAPDH is used as a positive control in SLP-76 (phospho Tyr128) Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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