SKP2 Cell Based Kit Elisa (A103728)

Nom du produit
SKP2 Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This SKP2 Cell Based ELISA Kit allows for the detection of SKP2 and the effects that certain stimulation conditions have on SKP2 expression in different cell lines. Qualitative determination of SKP2 concentration is achieved by an indirect ELISA format. In essence, the SKP2 is captured by Anti-SKP2 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this SKP2 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
CDK2/Cyclin A associated protein p45, Cyclin A/CDK2 associated protein p45, Cyclin-A/CDK2-associated protein p45, F box protein Skp2, F box/LRR repeat protein 1, F-box protein Skp2, F-box/LRR-repeat protein 1, FBL 1, FBL1, FBXL 1, FBXL1, FLB 1, FLB1, MGC1366, p45, p45skp2, S phase kinase associated protein 2, S phase kinase associated protein 2 (p45), S-phase kinase-associated protein 2, S-phase kinase-associated protein 2 E3 ubiquitin protein ligase, SKP 2, SKP2_HUMAN
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

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