Anti-SIRP alpha Anticorps [BL1H7] (A279843)

$355

Anticorps monoclonal [BL1H7] de souris contre SIRP alpha pour WB, IHC-P, IP, Flow Cytometry et IHC-Fr.

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Frais de livraison
Date de livraison
Livraison sous 4-6 jours ouvrables.
Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com
Nom du produit
Anti-SIRP alpha Antibody [BL1H7]
Description du produit
Mouse monoclonal [BL1H7] antibody to SIRP alpha.
Spécificité
This antibody recognises porcine CD172a, a member of the signal regulatory protein (SIRP) family. Mouse anti Pig CD172a, clone BL1H7 was originally clustered as SWC3 at the Third International Swine Cluster of Differentiation Workshop (Haverson et al. 2001;Thacker et al. 2001). CD172a is expressed on monocyte derived dendritic cells (MoDCs) also conventional (cDCs), plasmacytoid (pDCs) DCs and blood DCs.(Facci; Jeong et al. 2010). Mouse anti Pig CD172a, clone BL1H7 immunoprecipitates a single band of ~90-110 kDa from preparations of biotinylated alveolar macrophages, a result confirmed by Western blotting analysis of alveolar macrophage lysates under non reducing conditions. Aberrant expression of CD172a has been noted on porcine leukemias with blast cells co-expressing lymphocytic markers CD5 and CD25 whilst expressing the Myeloid marker CD172a in a bi-phenotypic pattern as opposed to the more characteristic single population of CD172+ cells seen in normal blood PBMC. Mouse anti Pig CD172a, clone BL1H7 has proved a useful and reliable tool for immunohistochemical analysis of routinely processed, formalin fixed, paraffin embedded porcine tissues.
Applications
WB, IHC-P, IP, Flow Cytometry, IHC-Fr
Recommander des dilutions
Flow Cytometry: 1:10 - 1:20, Use 10µl of the suggested working dilution to 1x106 cells in 100µl
Reactivité
Porcine
Immunogène
Porcine alveolar macrophages.
Hôte
Mouse
Clonalité
Monoclonal
Clone
BL1H7
Isotype
IgG1
Conjuguer

Unconjugated

Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Masse moléculaire
Approximately 90-115 kDa in alveolar macrophage lysates.
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Stockage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
Notes générales
Mouse anti Pig CD172a, clone BL1H7 recognizes porcine CD172a, a member of the signal regulatory protein (SIRP) family (Alvarez et al. 2000).Mouse anti Pig CD172a, clone BL1H7 was originally clustered as SWC3 at the Third International Swine Cluster of Differentiation Workshop (Haverson et al. 2001;Thacker et al. 2001). CD172a is expressed on monocyte derived dendritic cells (MoDCs) (Facci et al. 2010) also conventional (cDCs), plasmacytoid (pDCs) DCs and blood DCs.(Facci; Jeong et al. 2010). Mouse anti Pig CD172a, clone BL1H7 immunoprecipitates a single band of ~90-110 kDa from preparations of biotinylated alveolar macrophages, a result confirmed by Western blotting analysis of alveolar macrophage lysates under non reducing conditions (Alvarez et al. 2000). Aberrant expression of CD172a has been noted on porcine leukemias (Sipos et al. 2006) with blast cells co-expressing lymphocytic markers CD5 and CD25 whilst expressing the Myeloid marker CD172a in a bi-phenotypic pattern as opposed to the more characteristic single population of CD172+ cells seen in normal blood PBMC (Chamorro et al. 2005).Mouse anti Pig CD172a, clone BL1H7 has proved a useful and reliable tool for immunohistochemical analysis of routinely processed, formalin fixed, paraffin embedded porcine tissues (Domenech et al. 2003).
Synonymes
Bit, Brain Ig like molecule with tyrosine based activation motifs, Brain Ig-like molecule with tyrosine-based activation motifs, Brain immunoglobulin like molecule with tyrosine based activation motifs, CD172 antigen like family member A, CD172 antigen-like family member A, CD172a, CD172a antigen, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MFR, MYD 1, Myd 1 antigen, MyD-1 antigen, p84, Protein tyrosine phosphatase non receptor type substrate 1, PTPNS1, SHP substrate 1, SHPS-1, SHPS1, SHPS1_HUMAN, Signal regulatory protein alpha, Signal regulatory protein alpha 2, Signal regulatory protein alpha 3, Signal regulatory protein alpha type 1, Signal regulatory protein alpha type 2, Signal-regulatory protein alpha-1, Signal-regulatory protein alpha-2, Signal-regulatory protein alpha-3, SIRP, Sirp-alpha-1, Sirp-alpha-2, Sirp-alpha-3, SIRPA, SIRPalpha, SIRPalpha1, SIRPalpha2, SIRPalpha3, Tyrosine phosphatase SHP substrate 1, Tyrosine protein phosphatase non receptor type substrate 1, Tyrosine-protein phosphatase non-receptor type substrate 1
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

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