Anti-SHP2 (phospho Tyr542) Anticorps (A16556)

Name: Anti-SHP2 (phospho Tyr542) Antibody
Brand: Antibodies.com
SKU: A16556
Price: 435.0 USD
Availability: InStock

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$435

Anticorps polyclonal de lapin contre SHP2 (phospho Tyr542) pour WB et IHC.

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Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-SHP2 (phospho Tyr542) Antibody

Description du produit

Rabbit polyclonal antibody to SHP2 (phospho Tyr542).

Applications

WB, IHC

Recommander des dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:100

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic phosphorylated peptide around Y542 of human PTPN11 (NP_002825.3).

Séquence

HEYTN

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

72 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

BPTP3, CFC, JMML, METCDS, MGC14433, NS1, OTTHUMP00000166107, OTTHUMP00000166108, Protein tyrosine phosphatase 2, Protein tyrosine phosphatase 2C, Protein tyrosine phosphatase non receptor type 11, Protein-tyrosine phosphatase 1D, Protein-tyrosine phosphatase 2C, PTN11_HUMAN, PTP-1D, PTP-2C, PTP1D, PTP2C, PTPN11, SAP2, SH-PTP2, SH-PTP3, SH2 domain containing protein tyrosine phosphatase 2, SHP 2, SHP-2, SHPTP2, SHPTP3, Syp, Tyrosine-protein phosphatase non-receptor type 11

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Western Blot - Anti-SHP2 (phospho Tyr542) Antibody (A16556)

Western blot analysis of NIH/3T3, using Anti-SHP2 (phospho Tyr542) Antibody (A16556) at 1:700 dilution. NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Western Blot - Anti-SHP2 (phospho Tyr542) Antibody (A16556)

Western blot analysis of C6, using Anti-SHP2 (phospho Tyr542) Antibody (A16556) at 1:700 dilution. C6cells were treated by PDGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-SHP2 (phospho Tyr542) Antibody (A16556)

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-SHP2 (phospho Tyr542) Antibody (A16556). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.