Principe du test
Sheep Holo TC (Holotranscobalamin) ELISA Kit (A326617) employs the competitive enzyme immunoassay technique for the quantitative measurement of sheep Holo TC (Holotranscobalamin) in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The 96-well microtiter plate has been pre-coated with Holo TC (Holotranscobalamin) antigen. During the incubation, Holo TC (Holotranscobalamin) present in the samples or standards competes with the fixed amount of immobilized Holo TC (Holotranscobalamin) for binding sites on the Biotinylated Anti-Holo TC (Holotranscobalamin) Antibody. The more Holo TC (Holotranscobalamin) present in a sample or standard, the less Biotinylated Anti-Holo TC (Holotranscobalamin) Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Holo TC (Holotranscobalamin) Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Holo TC (Holotranscobalamin) present in each sample or standard. The concentration of Holo TC (Holotranscobalamin) can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.