Anti-SF3B4 Antibody [3A1] (A85417)

Human HeLa cells stained with Anti-SF3B4 Antibody (red), Anti-Vimentin Antibody (A85421 | green) and DNA (blue, stained with DAPI). The Anti-SF3B4 Antibody reveals strong granular nuclear staining which is a little different from the DNA stain and presumably reflects splicosomal complexes. The Anti-Vimentin Antibody stains the cytoplasmic intermediate filament network of the HeLa cells.

Immunofluorescent analysis of HeLa cells stained with Anti-SF3B4 Antibody [3A1] (A85417), at a dilution of 1:1,000, in red, and co-stained with Anti-Vimentin Antibody (A85421), at a dilution of 1:10,000, in green. The blue is DAPI staining of nuclear DNA. The Anti-SF3B4 Antibody [3A1] (A85417) reveals strong granular staining of the nuclei, while the Anti-Vimentin Antibody (A85421) specifically labels cytoplasmic intermediate filaments.

Western blot analysis of different cell lysates, cytosol or nuclear enriched fractions, using Anti-SF3B4 Antibody [3A1] (A85417), at a dilution of 1:1,000, in green. The lanes contain samples of: [1] Protein standards, in red, [2] NIH-3T3 cytosolic fraction, [3] NIH-3T3 nuclear fraction, [4] HeLa cytosolic, and [5] HeLa nuclear fractions. The strong single band at 49 kDa represents the SF3B4 protein, which is expressed exclusively in the nuclei. The same blot was simultaneously probed with Anti-GAPDH Antibody (A85377), at a dilution of 1:20,000, in red. The 37 kDa band corresponds to the GAPDH protein, detected mainly in the cytosolic fractions of these cells.

Blots of HeLa cell crude extract stained with Anti-SF3B4 Antibody. SF3B4 runs with an apparent SDS-PAGE molecular weight 49 kDa.