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SENP8 Cell Based Kit Elisa (A103114)

Nom du produit
SENP8 Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This SENP8 Cell Based ELISA Kit allows for the detection of SENP8 and the effects that certain stimulation conditions have on SENP8 expression in different cell lines. Qualitative determination of SENP8 concentration is achieved by an indirect ELISA format. In essence, the SENP8 is captured by Anti-SENP8 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this SENP8 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
cysteine 2, DEN 1, DEN1, Deneddylase 1, Deneddylase-1, FKSG8, HsT17512, NEDD 8 specific protease 1, NEDD8 specific protease cysteine 2, NEDD8-specific protease 1, NEDP 1, NEDP1, Protease, Protease, cysteine 2, PRSC2, SENP8_HUMAN, Sentrin / SUMO specific protease SENP 8, Sentrin-specific protease 8, Sentrin/SUMO-specific protease SENP8, SUMO sentrin specific protease family member 8, SUMO/sentrin peptidase family member, NEDD8 specific, SUMO/sentrin specific peptidase family member 8
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - SENP8 Cell Based ELISA Kit (CB5624) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - SENP8 Cell Based ELISA Kit (CB5624)
Western blot analysis of extracts from HuvEc cells, using Anti-SENP8 Antibody.
Protocol Diagram - SENP8 Cell Based ELISA Kit (CB5624) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: SENP8 Cell Based ELISA Kit (CB5624)
A diagram showing the protocol for SENP8 Cell Based ELISA Kit.
Protocol Summary - SENP8 Cell Based ELISA Kit (CB5624) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: SENP8 Cell Based ELISA Kit (CB5624)
A summary of the protocol for SENP8 Cell Based ELISA Kit.
Western Blot - Control Antibody - SENP8 Cell Based ELISA Kit (CB5624) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - SENP8 Cell Based ELISA Kit (CB5624)
The mouse monoclonal antibody to GAPDH is used as a positive control inSENP8 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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