Anti-S6K1 (phospho Thr421 + Ser424) Anticorps [ARC0107] (A10982)

Name: Anti-S6K1 (phospho Thr421 + Ser424) Antibody [ARC0107]
Brand: Antibodies.com
SKU: A10982
Price: 540.0 USD
Availability: InStock

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$540

Anticorps monoclonal [ARC0107] de lapin contre S6K1 (phospho Thr421 + Ser424) pour WB, IHC et ICC/IF.

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Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

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Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Mots clés :

Ribosomal Marker

Spécifications

Nom du produit

Anti-S6K1 (phospho Thr421 + Ser424) Antibody [ARC0107]

Description du produit

Rabbit monoclonal [ARC0107] antibody to S6K1 (phospho Thr421 + Ser424).

Applications

WB, IHC, ICC/IF

Recommander des dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivité

Human, Mouse, Rat

Immunogène

A synthesized peptide derived from human Phospho-S6K1 (T421 + S424).

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC0107

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

70 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

70 kDa ribosomal protein S6 kinase 1, KS6B1_HUMAN, p70 alpha, P70 beta 1, p70 ribosomal S6 kinase alpha, p70 ribosomal S6 kinase beta 1, P70 S6 Kinase, p70 S6 kinase alpha, p70 S6 kinase, alpha 1, p70 S6 kinase, alpha 2, p70 S6K, p70 S6K-alpha, p70 S6KA, p70(S6K) alpha, p70(S6K)-alpha, p70-alpha, p70-S6K, p70-S6K 1, P70S6K, P70S6K1, p70S6Kb, PS6K, Ribosomal protein S6 kinase 70kDa polypeptide 1, Ribosomal protein S6 kinase beta 1, Ribosomal protein S6 kinase beta-1, Ribosomal protein S6 kinase I, RPS6KB1, S6K, S6K-beta-1, Serine/threonine kinase 14 alpha, Serine/threonine-protein kinase 14A, STK14A

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (7)

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Figure 1: Western Blot - Anti-S6K1 (phospho Thr421 + Ser424) Antibody [ARC0107] (A10982)

Western blot analysis of extracts of various cell lines, using Anti-S6K1 (phospho Thr421 + Ser424) Antibody [ARC0107] (A10982) at 1:1,000 dilution. NIH/3T3 and C6 cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnightThe secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60S.

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Figure 2: Immunohistochemistry - Anti-S6K1 (phospho Thr421 + Ser424) Antibody [ARC0107] (A10982)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-S6K1 (phospho Thr421 + Ser424) Antibody [ARC0107] (A10982) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-S6K1 (phospho Thr421 + Ser424) Antibody [ARC0107] (A10982)

Immunohistochemistry analysis of paraffin-embedded human oophoroma using Anti-S6K1 (phospho Thr421 + Ser424) Antibody [ARC0107] (A10982) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-S6K1 (phospho Thr421 + Ser424) Antibody [ARC0107] (A10982)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-S6K1 (phospho Thr421 + Ser424) Antibody [ARC0107] (A10982) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.