Anti-S100A9 Anticorps (A84636)

Name: Anti-S100A9 Antibody
Brand: Antibodies.com
SKU: A84636
Price: 480.0 USD
Availability: InStock

Ce produit est identique à Novus (NBP1-18843). Découvrez plus pour moins cher.

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$480

Anticorps polyclonal de chèvre contre S100A9 pour ELISA, WB, IHC, IF et Flow Cytometry.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 3-5 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-S100A9 Antibody

Description du produit

Goat polyclonal antibody to S100A9.

Applications

ELISA, WB, IHC, IF, Flow Cytometry

Recommander des dilutions

ELISA: 1:8,000, WB: 0.5-2µg/ml, IHC: 2.5ug/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivité

Human

Immunogène

Synthetic peptide corresponding to the internal region of human S100A9.

Séquence

C-DTNADKQLSFEEF

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Masse moléculaire

13-14 kDa

MW prédit

13.2kDa

Forme du produit

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

60B8AG, CAGB, Calgranulin B, Calgranulin-B, Calprotectin L1H subunit, CFAG, CGLB, Cystic fibrosis antigen B, L1AG, Leukocyte L1 complex heavy chain, LIAG, MAC387, MIF, Migration inhibitory factor related protein 14, Migration inhibitory factor-related protein 14, MRP 14, MRP-14, MRP14, Myeloid-related protein 14, NIF, OTTHUMP00000015331, p14, Protein S100-A9, S100 A9, S100 calcium binding protein A9, S100 calcium binding protein A9 calgranulin B, S100 calcium-binding protein A9, S10A9_HUMAN

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (7)

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Figure 1: Western Blot - Anti-S100A9 Antibody (A84636)

S100A9 expression in Human Bone Marrow (A), Gastrointestinal cancer (B), and negative control HepG2 (C) lysate analyzed by western blot. Cells were lysed in RIPA buffer and 5µg protein was run per lane. Primary antibody was performed with Anti-S100A9 Antibody (A84636) at 1µg/ml (A) and 0.5µg/ml (B) and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-S100A9 Antibody (A84636)

S100A9 expression in MCF7 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-S100A9 Antibody (A84636) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Immunofluorescence - Anti-S100A9 Antibody (A84636)

S100A9 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-S100A9 Antibody (A84636) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Flow Cytometry - Anti-S100A9 Antibody (A84636)

S100A9 expression in MCF7 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-S100A9 Antibody (A84636) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.