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Anti-RYK Antibody (A305053)

Western Blot - Anti-RYK Antibody (A305053) - Antibodies.com Immunohistochemistry - Anti-RYK Antibody (A305053) - Antibodies.com
$520
Product Datasheet

Rabbit polyclonal antibody to RYK for WB and IHC.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-RYK Antibody
Description
Rabbit polyclonal antibody to RYK.
Specificity
Band at 37 kDa is potential degradation product.
Applications
WB, IHC
Dilutions
WB: 1:1,000, IHC: 1:50
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide of Human RYK (150-250 aa), conjugated to Keyhole Limpet Haemocyanin (KLH)
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Peptide affinity purification.
Concentration
1 mg/ml
Molecular Weight
67.5 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
JTK5A, Tyrosine-protein kinase RYK
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-RYK Antibody (A305053) - Antibodies.comEnlarge Image

Western Blot - Anti-RYK Antibody (A305053)

Western blot analysis of mouse brain lysate showing detection of ~67.5 kDa RYK protein using Anti-RYK Antibody (A305053) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: mouse brain lysate. Load: 15 µg. Block: 5% Skim Milk in TBST. The secondary antibody used was Goat Anti-Rabbit HRP:IgG at 1:4,000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~67.5 kDa. Other Band(s): ~37 kDa degradation product.
Immunohistochemistry - Anti-RYK Antibody (A305053) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-RYK Antibody (A305053)

Immunohistochemistry analysis of human breast cancer, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-RYK Antibody (A305053) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
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