Figure 1: Western Blot - Anti-RPGR Antibody (A13696)
Western blot analysis of extracts of Rat testis, using Anti-RPGR Antibody (A13696) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Figure 2: Western Blot - Anti-RPGR Antibody (A13696)
Western blot analysis of extracts of Mouse brain, using Anti-RPGR Antibody (A13696) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-RPGR Antibody (A13696) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-RPGR Antibody (A13696) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-RPGR Antibody (A13696) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of mouse retina cells using Anti-RPGR Antibody (A13696) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of rat retina cells using Anti-RPGR Antibody (A13696) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
