Anti-ROR gamma Anticorps (A82850)

Name: Anti-ROR gamma Antibody
Brand: Antibodies.com
SKU: A82850
Price: 480.0 USD
Availability: InStock

Ce produit est identique à Abcam (ab113434). Découvrez plus pour moins cher.

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$480

Anticorps polyclonal de chèvre contre ROR gamma pour ELISA, WB, IF et Flow Cytometry.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 3-5 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-ROR gamma Antibody

Description du produit

Goat polyclonal antibody to ROR gamma.

Applications

ELISA, WB, IF, Flow Cytometry

Recommander des dilutions

ELISA: 1:16,000, WB: 0.01-0.1µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivité

Human

Immunogène

Synthetic peptide corresponding to the internal region, amino acids 200-212, of human ROR gamma.

Séquence

CHLEYSPERGKAE

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Masse moléculaire

55-60 kDa

MW prédit

55.8kDa

Forme du produit

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

IMD42, MGC129539, NR1F3, Nuclear receptor ROR gamma, Nuclear receptor ROR-gamma, Nuclear receptor RZR gamma, Nuclear receptor RZR-gamma, Nuclear receptor subfamily 1 group F member 3, RAR related orphan nuclear receptor variant 2, RAR related orphan receptor C, RAR related orphan receptor C, isoform a, RAR related orphan receptor gamma, RAR-related orphan receptor C, Retinoic acid binding receptor gamma, Retinoid related orphan receptor gamma, Retinoid-related orphan receptor-gamma, Rorc, RORG, RORG_HUMAN, RZR GAMMA, RZRG, TOR

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Western Blot - Anti-ROR gamma Antibody (A82850)

ROR gamma expression in Human Colon (A), Liver (B), and Testes (C) lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-ROR gamma Antibody (A82850) at 0.1µg/ml (A), 0.03µg/ml (B), or 0.01µg/ml (C) and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-ROR gamma Antibody (A82850)

ROR gamma expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-ROR gamma Antibody (A82850) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic, plasma membrane, and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Flow Cytometry - Anti-ROR gamma Antibody (A82850)

ROR gamma expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-ROR gamma Antibody (A82850) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.