Anti-RNF13 Antibody (A83667)

Name: Anti-RNF13 Antibody
Brand: Antibodies.com
SKU: A83667
Price: 495.0 USD
Availability: InStock

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$495

Goat polyclonal antibody to RNF13 for ELISA, WB, IHC and Flow Cytometry.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 4-6 business days.

Specifications

Name

Anti-RNF13 Antibody

Description

Goat polyclonal antibody to RNF13.

Applications

ELISA, WB, IHC, Flow Cytometry

Dilutions

ELISA: 1:1,000, WB: 1-3µg/ml, IHC: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the C terminal region of human RNF13 (Isoform 1 and 3).

Sequence

C-PNGERDYNIANTV

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

42 kDa

Predicted MW

42.8kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

E3 ubiquitin-protein ligase RNF13, RING finger protein 13, RZF

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-RNF13 Antibody (A83667)

RNF13 expression in A431 (A) and HeLa (B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-RNF13 Antibody (A83667) at 2µg/ml and detected by chemiluminescence.

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Flow Cytometry - Anti-RNF13 Antibody (A83667)

RNF13 expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-RNF13 Antibody (A83667) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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Immunohistochemistry - Anti-RNF13 Antibody (A83667)

RNF13 expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-RNF13 Antibody (A83667) at 5µg/ml and revealed with horseradish peroxidase (HRP).

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Immunohistochemistry - Anti-RNF13 Antibody (A83667)

Negative control for RNF13 expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.

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