Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Ser2) Anticorps (A88927)

Western blot analysis of extracts of 293T cells, using Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Ser2) Antibody (A88927) at 1:1,000 dilution. 293T cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour or treated by Doxorubicin (0. 5uM) for 24 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.

Western blot analysis of extracts of 293T cells, using Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Ser2) Antibody (A88927) at 1:1,000 dilution or POLR2A antibody (A2107). 293T cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.

Immunoprecipitation analysis of 200µg extracts of MCF-7 cells using 3µg of Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Ser2) Antibody (A88927). This Western blot was performed on the immunoprecipitate using Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Ser2) Antibody (A88927) at a dilution of 1:1000. MCF7 cells were treated by nocodazole (50 ng/ml) at 37°C for 20 hours.