The protein has a predicted molecular mass of 51.3 kDa after removal of the signal peptide. The apparent molecular mass of M-CSF-hFc is approximately 55-70 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 95% by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Recombinant Human M-CSF Protein (Fc Tag) (A318188)
ELISA plates were pre-coated with Recombinant Human CSF-1-R Protein (6×His Tag) (A318346) at 2 µg/ml (100 µl/well) which can bind Recombinant Human M-CSF Protein (Fc Tag) (A318188) in a linear range of 0.32-200 ng/ml.
Recombinant Human M-CSF Protein (Fc Tag) (A318188)
ELISA plates were pre-coated with Recombinant Human M-CSF Protein (Fc Tag) (A318188) at 2 µg/ml (100 µl/well) which can bind Lacnotuzumab Biosimilar - Anti-M-CSF Antibody - BSA and Azide free (A318861) in a linear range of 9.77-156.25 ng/ml.
Flow Cytometry - Recombinant Human M-CSF Protein (Fc Tag) (A318188)
Flow cytometry analysis of 1µg/ml of Recombinant Human M-CSF Protein (Fc Tag) (A318188) on Expi293 cells transfected with human CSF1R (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
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