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Recombinant Bet v 2 (2.0101) Protéine (A242917)

SDS-PAGE - Recombinant Bet v 2 (2.0101) Protein (A242916) - Antibodies.com Flow Cytometry - Recombinant Bet v 2 (2.0101) Protein (A242917) - Antibodies.com Flow Cytometry - Recombinant Bet v 2 (2.0101) Protein (A242917) - Antibodies.com
$525
Product Datasheet
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Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 4-6 jours ouvrables.

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Nom du produit
Recombinant Bet v 2 (2.0101) Protein
Applications
ELISA, Flow Cytometry, WB
Système d'expression
Escherichia coli
Nature
Recombinant
Espèces de protéines
Betula verrucosa (Silver Birch)
Séquence
DNA sequence encoding 152 amino acids was fused with a Strep Tag at the N-terminus to form this recombinant protein.
Étiquette
Strep Tag (N-terminus)
MW prédit
16.2 kDa
Conjuguer

Unconjugated

Purification
Ion exchange and affinity chromatography.
Forme du produit
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from 100mM Tris Buffer, pH 8, with 150mM NaCl, and without preservatives or carriers (0.2 µm filter sterilized).
Stockage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Use immediately or aliquot and store at -20°C to -80°C. Avoid freeze/thaw cycles.
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
SDS-PAGE - Recombinant Bet v 2 (2.0101) Protein (A242916) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: SDS-PAGE - Recombinant Bet v 2 (2.0101) Protein (A242917)
Purity verification: 5 µg of Recombinant Bet v 2 (2.0101) Protein with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.
Flow Cytometry - Recombinant Bet v 2 (2.0101) Protein (A242917) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Flow Cytometry - Recombinant Bet v 2 (2.0101) Protein (A242917)
Flow cytometry dot-plot staining pattern of Recombinant Bet v 2 (2.0101) Protein stimulated human peripheral whole blood lymphocytes and basophils of a proven allergic donor stained using Anti-CD63 Antibody [MEM-259] (FITC) and Anti-CD203c Antibody [NP4D6] (PE).
Flow Cytometry - Recombinant Bet v 2 (2.0101) Protein (A242917) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Flow Cytometry - Recombinant Bet v 2 (2.0101) Protein (A242917)
An ELISA test was designed to prove the bond between the coated target, Recombinant Bet v 2 (2.0101) Protein, and allergen-specific human plasma IgG4 antibodies of Betula verrucosa positive donor. A measurable signal was subsequently generated by the addition of Anti-Human IgG4 Antibody (Biotin), Streptavidin-HRP, and substrate solution (TMB). The intensity of the signal is proportional to the amount of coated Recombinant Bet v 2 (2.0101) Protein.
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