Rat IL1R1 Kit Elisa (A327114)

Rat IL1R1 ELISA Kit

Rat IL1R1 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay designed for the in vitro quantitative determination of rat IL1R1 in serum, plasma, and other biological fluids.

Sandwich (quantitative)

Rat IL1R1 ELISA Kit (A327114) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat IL1R1 in serum, plasma, and other biological fluids. An antibody specific for IL1R1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the IL1R1 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-IL1R1 Antibody, which binds the captured IL1R1 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of IL1R1 captured in each well. The concentration of IL1R1 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colormetric

Colorimetric Microplate Reader

Serum, plasma, and other biological fluids.

46.88 pg/ml

78.13-5000 pg/ml

3h 30m

No significant cross-reactivity or interference between rat IL1R1 and analogues was observed.

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Information online is representative. Always refer to the Product Manual inside the kit.

Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.

Antigen CD121a, CD121 antigen-like family member A, CD121a, CD121a antigen, D2S1473, IL 1, IL 1R 1, IL 1R alpha, IL 1RT1, IL-1R-1, IL-1R-alpha, IL-1RT-1, IL-1RT1, IL1 inhibitor, IL1 Receptor I, IL1R, IL1R1_HUMAN, IL1RA, IL1RN, IL1RT1, Interleukin 1 receptor alpha type 1, Interleukin 1 receptor antagonist protein (IRAP), Interleukin 1 receptor type I, Interleukin receptor 1, Interleukin-1 receptor alpha, Interleukin-1 receptor type 1, Interleukin-1 receptor type I, p80