Rat IL-2 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of rat IL-2 in the samples. A monoclonal antibody specific for rat IL-2 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for IL-2, and IL-2 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-2 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Cell culture supernatant, serum and plasma.
Sensibilité
1.38 pg/ml
Gamme de detection
31.25 - 2000 pg/ml
Récupération
102% (Average Recovery from Serum Samples).
Spécificité
This kit can assay both natural and recombinant rat IL-2.
Reactivité
Rat
Réactivité croisée
This assay has shown no significant cross-reactivity.
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at 2 - 8°C.
Synonymes
Aldesleukin, IL 2, IL2, IL2_HUMAN, Interleukin 2, Interleukin-2, Interleukin2, Involved in regulation of T cell clonal expansion, Lymphokine , OTTHUMP00000164090, POIL2, T cell growth factor, T-cell growth factor, TCGF
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
