Rat IL-10 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of rat IL-10 in the samples. A monoclonal antibody specific for rat IL-10 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for IL-10, and IL-10 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-10 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Cell culture supernatant, serum and plasma.

Sensibilité

0.48 pg/ml

Gamme de detection

31.25 - 2000 pg/ml

Récupération

98% (Average Recovery from Serum Samples).

Spécificité

This kit can assay both natural and recombinant rat IL-10.

Reactivité

Rat

Réactivité croisée

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2 - 8°C.

Synonymes

CSIF, Cytokine synthesis inhibitory factor, GVHDS, IL 10, IL10, IL10A, IL10_HUMAN, Interleukin 10 , Interleukin-10, Interleukin10, MGC126450, MGC126451, T cell growth inhibitory factor, T-cell growth inhibitory factor, TGIF

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.