Rat Factor H Kit Elisa (A327115)

Rat Factor H ELISA Kit

Rat Factor H ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay designed for the in vitro quantitative determination of rat Factor H in serum, plasma, and other biological fluids.

Sandwich (quantitative)

Rat Factor H ELISA Kit (A327115) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat Factor H in serum, plasma, and other biological fluids. An antibody specific for Factor H has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Factor H present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Factor H Antibody, which binds the captured Factor H present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Factor H captured in each well. The concentration of Factor H can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colormetric

Colorimetric Microplate Reader

Serum, plasma, and other biological fluids.

37.50 ng/ml

62.50-4000 ng/ml

3h 30m

No significant cross-reactivity or interference between rat Factor H and analogues was observed.

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Information online is representative. Always refer to the Product Manual inside the kit.

Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.

adrenomedullin binding protein, age related maculopathy susceptibility 1, AHUS 1, AHUS1, AMBP 1, AMBP1, ARMD 4, ARMD4, ARMS 1, ARMS1, beta 1 H globulin, beta 1H, beta1H, CFAH_HUMAN, CFH, CFHL 3, CFHL3, Complement factor H, complement factor H, isoform b, factor H like 1, FH, FHL 1, FHL1, H factor 1, H factor 1 (complement), H factor 2 (complement), HF, HF 1, HF 2, HF1, HF2, HUS, MGC88246