Rat CXCL2 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of rat CXCL2 in the samples. A monoclonal antibody specific for rat CXCL2 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for CXCL2, and CXCL2 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of CXCL2 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Cell culture supernatant, serum and plasma.

Sensibilité

1.84 pg/ml

Gamme de detection

31.25 - 2000 pg/ml

Récupération

101% (Average Recovery from Serum Samples).

Spécificité

This kit can assay both natural and recombinant rat CXCL2.

Reactivité

Rat

Réactivité croisée

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2 - 8°C.

Synonymes

C-X-C motif chemokine 2, Chemokine (C X C motif) ligand 2, Chemokine (C-X-C motif) ligand 2, Chemokine, CXC motif, ligand 2, CINC 2a, CINC-2a, CINC2a, CINC3, CXC chemokine, CXCL 2, CXCL2_HUMAN, Cytokine-induced neutrophil chemoattractant 3, GRO 2, GRO b, Gro beta, GRO protein, beta, Gro-beta, GRO-beta(5-73), GRO-beta-T, GRO2, GRO2 oncogene, GROb, GRObeta, Growth regulated protein beta, Growth-regulated protein beta, GROX, Hematopoietic synergistic factor, HSF, Macrophage inflammatory protein 2, Macrophage inflammatory protein 2 alpha, Macrophage inflammatory protein 2-alpha, Melanoma growth stimulatory activity beta, MGSA b, MGSA beta, MGSA-b, MIP 2, MIP 2a, MIP2, MIP2 alpha, MIP2-alpha, MIP2A, MIP2alpha, SB-251353, Scyb, SCYB 2, SCYB2, Small inducible cytokine subfamily B, member 2

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.