Nous Contacter
+1 (314) 370-6046 ouNous Contacter
Visit our English Website
Antibodies.com LogoAntibodies.com Logo
Se connecter / S'inscrireMon Compte
0

Rat CamKII gamma Kit Elisa (A2635)

Standard Curve - Rat CamKII gamma ELISA Kit (A2635) - Antibodies.com
$595
Qualité 100 % Garantie
Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 7-10 jours ouvrables.

Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com

Nom du produit
Rat CamKII gamma ELISA Kit
Description du produit
Rat CamKII gamma ELISA Kit is a sandwich (quantitative) Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat CamKII gamma in tissue homogenates, cell lysates or other biological fluids.
Type de test
Sandwich (quantitative)
Principe du test
Rat CamKII gamma ELISA Kit employs the Sandwich Enzyme Immunoassay (ELISA) technique for the quantitative measurement of rat CamKII gamma in rat. An antibody specific for CamKII gamma has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the CamKII gamma present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-CamKII gamma Antibody, which binds the captured CamKII gamma present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and Avidin conjugated to Horseradish Peroxidase (Avidin-HRP) is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of CamKII gamma captured in each well. The concentration of CamKII gamma can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
Plate-forme
Pre-coated Microplate (12 x 8 well strips)
Méthode de détection
Colourmetric
Instrument
Colorimetric Microplate Reader
Type d'échantillon
Tissue homogenates, cell lysates or other biological fluids.
Sensibilité
0.123 ng/ml
Gamme de detection
0.312-20 ng/ml
Durée du test
1 - 4.5 Hours
Reactivité
Rat
Recovery
Sample Type n Range Average
Serum 5 83% - 100% 91%
EDTA Plasma 5 90% - 98% 94%
Heparin Plasma 5 90% - 99% 94%
Linearity
Sample Type 1:2 1:4 1:8 1:16
Serum (n=5) 90-99% 82-108% 82-96% 92-103%
EDTA Plasma (n=5) 84-105% 88-94% 89-107% 72-106%
Heparin Plasma (n=5) 89-92% 88-108% 84-102% 90-98%
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Notes générales
Information online is representative. Always refer to the Product Manual inside the kit.
Stockage
Store at +4°C. Do not use past expiration date!
Synonymes
CaM kinase II, CaM kinase II gamma chain, CaM kinase II subunit gamma, CAMK, CAMK II, CaMK II subunit gamma, CAMK2G, CAMKG, CAMKII
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Standard Curve - Rat CamKII gamma ELISA Kit (A2635) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Standard Curve - Rat CamKII gamma ELISA Kit (A2635)
Representative standard curve for Rat CamKII gamma ELISA Kit (A2635).
Menu Principal Nous Contacter 0Caisse
Haut