Rat Activin A Kit Elisa (A511)

$655
Frais de livraison
Date de livraison
Livraison sous 7-10 jours ouvrables.
Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com
Nom du produit
Rat Activin A ELISA Kit
Type de test
Sandwich (quantitative)
Principe du test
This Activin A enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-Activin A Antibody. Standards or samples are then added to the microtire plate wells and Activin A, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of Activin A present in the sample, Anti-Activin A Antibody (Biotin) is added to each well to "sandwich" the Activin A immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Activin A will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Serum, body fluids, tissue lysates, and cell culture supernatants.
Sensibilité
< 12 pg/ml
Gamme de detection
62.5-4000 pg/ml
Reactivité
Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at 2-8°C for 4 months or at -20°C for 8 months.
Synonymes
Activin beta A chain, Activin beta-A chain, ActivinA, Beta 1, EDF, erythroid differentiation factor, erythroid differentiation protein, follicle stimulating hormone releasing protein, FSH releasing protein, INHBA, INHBA_HUMAN, Inhibin, inhibin beta A chain
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Haut